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KMID : 0545120230330050687
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Journal of Microbiology and Biotechnology
2023 Volume.33 No. 5 p.687 ~ p.697
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Unraveling Biohydrogen Production and Sugar Utilization Systems in the Electricigen Shewanella marisflavi BBL25
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Kim Sang-Hyun
Kim Hyun-Joong
Kim Su-Hyun
Jung Hee-Ju
Kim Byung-Chan
Cho Do-Hyun
Jeon Jong-Min
Yoon Jeong-Jun
Kim Sang-Hyoun
Park Jeong-Hoon
Shashi Kant Bhatia
Yang Yung-Hun
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Abstract
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Identification of novel, electricity-producing bacteria has garnered remarkable interest because of the various applications of electricigens in microbial fuel cell and bioelectrochemical systems. Shewanella marisflavi BBL25, an electricity-generating microorganism, uses various carbon sources and shows broader sugar utilization than the better-known S. oneidensis MR-1. To determine the sugar-utilizing genes and electricity production and transfer system in S. marisflavi BBL25, we performed an in-depth analysis using whole-genome sequencing. We identified various genes associated with carbon source utilization and the electron transfer system, similar to those of S. oneidensis MR-1. In addition, we identified genes related to hydrogen production systems in S. marisflavi BBL25, which were different from those in S. oneidensis MR-1. When we cultured S. marisflavi BBL25 under anaerobic conditions, the strain produced 427.58 ± 5.85 μl of biohydrogen from pyruvate and 877.43 ± 28.53 μl from xylose. As S. oneidensis MR-1 could not utilize glucose well, we introduced the glk gene from S. marisflavi BBL25 into S. oneidensis MR-1, resulting in a 117.35% increase in growth and a 17.64% increase in glucose consumption. The results of S. marisflavi BBL25 genome sequencing aided in the understanding of sugar utilization, electron transfer systems, and hydrogen production systems in other Shewanella species.
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KEYWORD
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Shewanella, whole-genome sequencing, biohydrogen production, carbon source utilization system
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